EC HPLC column (analytical), NUCLEODUR 300-5 C4 ec, 5 µm, 100×3 mm
$33.99
Wide pore RP phase for daily routine analysis. The medium density butyl modification is based on high purity NUCLEODUR silica gel and is often used for the separation of biomolecules. Butyl (C4) modifications have a lower retention for hydrophobic compounds compared to C18 (octadecyl silane, ODS) or C8 (octyl, MOS)…
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Recommended application(s)
HPLC
Brand
NUCLEODUR
Base material
NUCLEODUR – high purity silica
Particle type
Fully porous particles (FPP)
Particle shape
Spherical
Mode
Reversed phase (RP)
Endcapped
Yes
Particle size
5 µm
Pore size
300 Å
Scope of delivery
1x analytical HPLC column, instruction leaflet, certificate of analysis
Temperature stability (max)
60 °C
Tmax (phosphate buffer)
40 °C
Method
HPLC, analytical
Hardware
Stainless steel
Type
EC HPLC column (analytical)
Pore volume
0.9 mL/g
Eluent in HPLC column
Acetonitrile – water
Column inner diameter
3 mm
Column length
100 mm
Column volume
0.7 mL
pH stability
1.0–9.0
Recommended application(s)
Biological macromolecules
Recommended application(s)
Proteins
Recommended application(s)
Peptides
Pressure stability (max)
450 bar
USP listing
L26
Phase
NUCLEODUR 300-5 C4 ec
Specific surface according to BET
100 m²/g
Carbon content
2.5 %
Surface chemistry
Medium density monomeric butyl (C4) wide-pore modification
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Macherey Nagel – Chromatography Catalogue
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