EC HPLC column (analytical), NUCLEODUR HILIC, 3 µm, 250×3 mm
$81.99
Separation science is always looking for new and effective strategies to accomplish the tasks of modern analytics. Especially for polar compounds reversed phase HPLC – the most common analytical method – is often limited. Here, hydrophilic stationary phases provide an additional tool for the separation of polar analytes in HPLC….
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Brand
NUCLEODUR
Pore size
110 Å
Specific surface according to BET
340 m²/g
Base material
NUCLEODUR – high purity silica
Particle type
Fully porous particles (FPP)
Particle shape
Spherical
Endcapped
No
USP listing
Not available or proprietary
Recommended application(s)
Amino acids
Scope of delivery
1x analytical HPLC column, instruction leaflet, certificate of analysis
Temperature stability (max)
60 °C
Tmax (phosphate buffer)
40 °C
Pressure stability (max)
600 bar
Method
HPLC, analytical
Hardware
Stainless steel
Type
EC HPLC column (analytical)
Particle size
3 µm
Pore volume
0.9 mL/g
Eluent in HPLC column
Acetonitrile – water
Column inner diameter
3 mm
Column length
250 mm
Column volume
1.75 mL
Recommended application(s)
Water-soluble vitamins
Recommended application(s)
Peptides
Carbon content
7.0 %
Recommended application(s)
HILIC applications HPLC
Recommended application(s)
Highly polar analytes
Recommended application(s)
Hydrophilic compounds
Recommended application(s)
Organic polar acids and bases
Recommended application(s)
Polar natural compounds
Recommended application(s)
Nucleosides
Recommended application(s)
Oligonucleotides
pH stability
2.0–8.5
Mode
Hydrophilic Interaction Chromatography (HILIC)
Phase
NUCLEODUR HILIC
Surface chemistry
Ammonium – sulfonic acid (zwitterionic) modification
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Macherey Nagel – Chromatography Catalogue
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