Bioneer Taq DNA Polymerase, 10X Reaction Buffer without MgCl2, 20 mM MgCl2 (2,000 Units)

Bioneer Taq DNA Polymerase, 10X Reaction Buffer without MgCl2, 20 mM MgCl2 (2,000 Units)

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Taq DNA polymerase is a thermostable polymerase derived from the gene of Thermus aquaticus YT1 expressed and purified from Escherichia coli . 

※This product is shipped in dry ice.

 

Features and Benefits

Efficiency & Sensitivity

High efficiency and sensitivity to the templates 

Optimized buffer delivery

Stable PCR reaction by providing buffers optimized for the enzyme 

Reproducibility

Reproducible results with uniform quality products for each batch by manufacturing under the ISO 9001 quality system.

 

Applications

• Real-time quantification of DNA and cDNA targets using dual probe and dsDNA binding dye
• Gene expression profiling
• Microbial & viral pathogen detection.

Specifications

5′ to 3′ exonuclease activity	Yes
3′ to 5′ exonuclease activity	No
3′ – A overhang	Yes
Fragment size	Up to 10 kb

Kit Content

Cat. No.	Taq DNA Polymerase (500 U)	10 x Reaction Buffer	10 mM dNTPs	20 mM MgCl2	Dilution Buffer
E-2011	100 µl	1 ml (with MgCl2)	1 ml	–	1 ml
E-2011-1	100 µl	1 ml (without MgCl2)	1 ml	1 ml	1 ml
E-2011-2	100 µl	1 ml (with MgCl2)	–	–	1 ml
E-2011-3	100 µl	1 ml (without MgCl2)	–	1 ml	1 ml

10X reaction buffer with (or without) MgCl₂: Tris-HCl, KCl, 15 mM MgCl₂, pH 9.0

Dilution buffer: 20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, Stablizers, 50% Glycerol, pH 8.0

10 mM dNTPs mix: 2.5 mM of each dNTP

 

Concentration

500 units (5 U/μl)

 

Storage Conditions

20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, Stablizers, 50% Glycerol pH 8.0.

 

Storage Temperature

-20°C

 

Unit Definition

One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 72℃.